Product: XF Base Medium
Minimal DMEM
(0 mM Glucose)
Part Number: 102353-100 (2 x 1 liter)
XF Base Medium is for use with Seahorse Bioscience XF instruments, XF Assay Kit cartridges and XF Cell Culture Microplates. XF Base Medium has been developed to allow customization of substrates in your assay medium.
XF assays require a non-buffered medium to accurately measure the extracellular acidification rate (ECAR), and proton production rate (PPR). The constituents are based on Dulbecco’s Modified Eagle’s Medium (DMEM). No sodium bicarbonate (buffering agent), glucose, glutamine/GlutaMAXTM, or sodium pyruvate is present allowing the user to specifically customize the assay medium for their experimental design.XF Base Medium Specifications
Volume:
1 L
Osmolality:
291 mOsm/Kg H2O
Endotoxin:
< 0.0052 EU/mL
Storage:
4°C
Shelf life:
18 months when stored at 4°C