This basal culture medium contains:
 Essential and non-essential amino acids
 Vitamins
 Other organic compounds
 Trace minerals
 Inorganic salts. 
 
Human Fibroblast Expansion Basal Medium does NOT contain:
 Antibiotics
 Antimycotics
 Hormones
 Growth factors
 Proteins
 
This medium is HEPES and bicarbonate buffered and is designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support the plating and long-term proliferation of human dermal fibroblasts, Human Fibroblast Expansion Basal Medium must be supplemented with Low Serum Growth Supplement (LSGS, Cat. No. S-003-10), or Low Serum Growth Supplement Kit (LSGS Kit, Cat. No. S-003-K).