Cyclin D3,周期素D3抗体

产品编号HZ-0660R
英文名称Cyclin D3
中文名称周期素D3抗体
别 名CCND3; CyclinD3; D3 type cyclin; G1 S specific cyclin D3; CCND3_HUMAN; G1/S-specific cyclin-D3.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 细胞周期蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
Cyclin D3,周期素D3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量32kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Cyclin D3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Cyclin D3,周期素D3抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]..

Function:
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Subunit:
Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either CDKN1A or CDKN2A.

Cyclin D3,周期素D3抗体Subcellular Location:
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.

Post-translational modifications:
Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Similarity:
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.

Gene ID:
896

Database links:
Entrez Gene: 540547 Cow
Entrez Gene: 896 Human
Entrez Gene: 12445 Mouse
Entrez Gene: 25193 Rat
Omim: 123834 Human
SwissProt: Q3MHH5 Cow
SwissProt: P30281 Human
SwissProt: P30282 Mouse
SwissProt: P48961 Rat
Unigene: 534307 Human
Unigene: 27291 Mouse
Unigene: 472101 Mouse
Unigene: 3483 Rat
Unigene: 54319 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞周期素是周期素依赖激酶的调节因子，不同的细胞周期素控制着细胞周期中不同的特定阶段。
周期素D3与周期素D1和D2一样，为细胞周期中G1期进入S期的一个重要调控因子，通过激活CdK4或CdK6等作用，促进DNA合成，加速细胞增殖，主要用于B细胞淋巴瘤、乳腺癌、头颈部鳞癌、食道癌、肝癌和肺癌等恶性肿瘤的研究。